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. 2012 Jun 20;287(33):27326–27334. doi: 10.1074/jbc.M112.366658

FIGURE 1.

FIGURE 1.

Identification of catalytically active NIK kinase domain constructs. A, NIK sequence motifs based on literature reports (16, 21). NRD, negative regulatory domain; NCR, non-catalytic region; BR, basic region; PRR, proline-rich repeat. B, schematic of a selected panel of truncated NIK constructs with the kinase domain shown as slate boxes. C, in vitro kinase phosphorylation assays. Left panel, NIK autophosphorylation and IKKα(K43A) phosphorylation by NIK. IKKα(K43A) and NIK construct 329–747(K429A) (329–747-KA) are inactive alanine mutants of the catalytic Lys-43 and Lys-429, respectively. Right panel, immunoblot (IB) analysis of total cell extracts. Positions of molecular mass standards (in kilodaltons) are shown on the left for both panels. IP, immunoprecipitate.