TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristics | Ref. or Source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− end A hsdR17 (rK− mK+) supE44 thi-1 recA1 gyr-A96 _80lacZM15 | 28 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F− proABlacIqZ_M15 Tn10) | Stratagene |
| S17–1 | hsdR pro recA, RP4–2 in chromosome Km::Tn7 (Tc::Mu) | 37 |
| BL21(λD3) | F− ompT hsdSB (rB− mB−) gal dcm (λD3) | Novagen |
| A. hydrophila strains | ||
| AH-3 | O34, wild type | 37 |
| AH-405 | AH-3, spontaneous RifR | 37 |
| AH-3ΔFlrA | AH-3 mutant unable produce polar flagellum, produces lateral flagella | 30 |
| AH-3ΔPseB | AH-3 pseB mutant in frame with pDM4 | This study |
| AH-3ΔPseF | AH-3 pseF mutant in frame with pDM4 | This study |
| Plasmids | ||
| pRK2073 | Helper plasmid, SpcR | 37 |
| pBAD33 | Arabinose-inducible expression vector, CmR | 27 |
| pBAD-PseB | pBAD33 with AH-3 pseB | This study |
| pBAD-PseF | pBAD33 with AH-3 pseF | This study |
| pBAD-PseBCj | pBAD33 with C. jejuni pseB | This study |
| pBAD-PseFCj | pBAD33 with C. jejuni pseF | This study |
| pDM4 | pir dependent with sacAB genes, oriR6K, CmR | 25 |
| pDM-PseB | pDM4 with AH-3 pseB fragment, CmR | This study |
| pDM-PseF | pDM4 with AH-3 pseF fragment, CmR | This study |