TABLE 2.
Assigned oligopeptides
Based on nanoelectrospray liquid chromatography MS/MS analysis of tryptic digests of the AH-3 polar and lateral flagellins.
| Observed Precursor m/z | Monoisotopic precursor m/z | Charge state of glycopeptide | Residues | Sequence of glycopeptide | Unmodified peptide [M+H]+ | Protein (strain) | Mass excess (Da) |
|---|---|---|---|---|---|---|---|
| 870.4 | 870.4 | [M + 2H]2 + | 170–184 | VTSVNTAISTASAAAa | 1363.6 | LafA (AH3) | 376 |
| 1034.4 | 1034.4 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 376 |
| 1062.1 | 1062.1 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1492 |
| 1088.4 | 1088.4 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1571 |
| 1093.1 | 1093.1 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1585 |
| 1120.4 | 1119.4 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1665 |
| 1124.7 | 1124.1 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1679 |
| 1151.4 | 1151.0 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1759 |
| 1155.5 | 1155.1 | [M + 3H]3 + | 94–109 | TLAQQSANGSNNTDDR | 1691.8 | FlaA/FlaB (AH3) | 1772 |
| 939.4 | 939.4 | [M + 2H]2 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 376 |
| 1031.0 | 1029.8 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1585 |
| 1057.4 | 1056.1 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1665 |
| 1062.0 | 1060.7 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1679 |
| 1083.7 | 1082.4 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1743 |
| 1088.4 | 1087.1 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1758 |
| 1632.1 | 1630.6 | [M + 2H]2 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1758 |
| 1092.7 | 1091.7 | [M + 3H]3 + | 160–174 | MTSAFTISGIASSTK | 1501.8 | FlaB (AH3) | 1772 |
| 1158.5 | 1157.4 | [M + 3H]3 + | 175–192 | GSATVATSASGEPYTVTK | 1726.9 | FlaB (AH3) | 1743 |
| 1743.9 | 1742.5 | [M + 2H]2 + | 175–192 | GSATVATSASGEPYTVTK | 1726.9 | FlaB (AH3) | 1758 |
| 1167.8 | 1166.8 | [M + 3H]3 + | 175–192 | GSATVATSASGEPYTVTK | 1726.9 | FlaB (AH3) | 1772 |
| 1003.4 | 1001.8 | [M + 3H]3 + | 193–206 | GTTAVTSTSVSSIK | 1338.7 | FlaB (AH3) | 1665 |
| 1020.0 | 1018.7 | [M + 3H]3 + | 193–206 | GTTAVTSTSVSSIK | 1338.7 | FlaB (AH3) | 1715 |
| 1029.1 | 1028.1 | [M + 3H]3 + | 193–206 | GTTAVTSTSVSSIK | 1338.7 | FlaB (AH3) | 1744 |
| 1033.9 | 1032.8 | [M + 3H]3 + | 193–206 | GTTAVTSTSVSSIK | 1338.7 | FlaB (AH3) | 1758 |
| 1038.4 | 1037.4 | [M + 3H]3 + | 193–206 | GTTAVTSTSVSSIK | 1338.7 | FlaB (AH3) | 1772 |
| 1151.1 | 1149.8 | [M + 3H]3 + | 207–223 | AASSAQLAMANLDFMIK | 1781.9 | FlaB (AH3) | 1665 |
| 1181.7 | 1180.5 | [M + 3H]3 + | 207–223 | AASSAQLAMANLDFMIK | 1781.9 | FlaB (AH3) | 1758 |
a Atypical tryptic cleavage product, sequence confirmed with peptide type y and b ion coverage.