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PspA interacts with TatA and TatE. C-terminally Strep-tagged TatA (upper gel) or TatE (lower gel) were purified from membrane fractions by Strep-tactin affinity chromatography, and fractions were analyzed by SDS-PAGE/Coomassie staining. F, flow-through (unbound material); W, last wash fraction; E1–4, elution fractions. The positions of TatA, TatE, and PspA bands are indicated on the right. Molecular weight markers are indicated on the left.
