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. 2012 Jun 11;287(33):27834–27842. doi: 10.1074/jbc.M112.374983

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — The TatA-PspA interaction depends on PspB and PspC, but not on PspF. A, detection of PspA in purifications of TatA-strep produced in the E. coli psp-deletion strains JWK1296 ( = ΔpspF)/pBW-tatA-strep, JWK1299 ( = ΔpspC)/pBW-tatA-strep and JWK1298 ( = ΔpspB)/pBW-tatA-strep. B, complementation of the PspA-TatA interaction in the pspC deletion strain JWK1299 by expression of pspC in trans. Upper panel, detection of PspA in the TatA-strep purification fractions. Lower panel, detection of PspC in the TatA-strep purification fractions. Note that PspC co-elutes in TatA-strep purifications, as does PspA. C, complementation of the PspA-TatA interaction in the pspB deletion strain JWK1298 by expression of pspB in trans. Upper panel, detection of PspA in the TatA-strep purification fractions. Lower panel, detection of PspB in the TatA-strep purification fractions. All panels show immunoblots with detections of the proteins indicated on the right side.