FIGURE 8.

X-ray structure of NorM and hMATE1 homology model with the molecular surface of the translocation cleft. A and B, view of the cytoplasmic face of NorM (A) and a schematic representation (B) of its helical organization. C and D, stereo views of the side (C) and the extracellular face (D) of the hMATE1 homology model. The N-terminal half is shaded in light blue, and the C-terminal half is colored light green. The N terminus is noted, and the C-terminal short helix is omitted for clarity. E and F, stereo views of the electrostatic surface rendering of the internal cavity of hMATE1 homology model, showing the side composed of transmembranes 1, 5, 6, 8, 9, and 10 (E) and the other side comprising transmembranes 2, 3, 4, 7, 11, and 12 (F). Positively charged areas are shaded in deep blue, negatively charged areas are shown in red, and neutral areas are colored light gray. Stereo images and electrostatics representation were generated by PyMOL.