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. 2012 Jun 21;287(33):27971–27982. doi: 10.1074/jbc.M112.386979

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Substituted cysteine accessibility method profiles for selected rbMate1 residues (single-Cys replacement in the Δ13C mutant). A, Western blots of the accessibility profiles for selected MATE1 residues. Each three-lane “triplet” displays immunoreactive rbMate1 pulled down through reaction with (from left to right) NHS-biotin (NB), MTS-biotin (MB), or MTS-biotin following permeabilization with saponin (MB/Perm; see text for discussion). Accessibility profiles mapped on the homology model for rbMate1. Blue residues were accessible to MTS-biotin from the extracellular solution; orange residues were accessible to MTS-biotin only after permeabilization of the plasma membrane.