FIGURE 10.

Localization of Cx32-WT and CMTX mutants in HeLa cells. HeLa cells were seeded on glass coverslips and immunostained for Cx32. Note that Cx32-WT and mutants (R219H, R230C, and F235C) assembled into gap junctions (white arrows). White dashed box within merge image was enlarged on the right to show the gap junctions at sites of cell-cell contact. The scrape line was located to the right of the imaged cells (white dashed line). Note also that some of the protein remains intracellular as vesicular puncta in HeLa cells. Magnification at ×63 oil using extended focus.