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. 2012 Jun 20;287(33):27771–27788. doi: 10.1074/jbc.M112.382572

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 13. — Cx32-WT and mutants, SAP97, and CaM co-IP studies using HeLa cell lysates. A, lysates of nontransfected and transfected (Cx32-WT and mutants) HeLa cells were incubated with antibodies directed against Cx32 (Cx32 IP) and probed for Cx32 and SAP97. B, lysates of nontransfected and transfected (Cx32-WT and mutants) HeLa cells were incubated with antibodies directed against CaM (CaM IP) and probed for Cx32 and CaM. Input is the HeLa lysate. Co-IP experiments were performed in triplicate. C, quantification of the data shown in A. SAP97 co-IP intensity was normalized to Cx32-WT IP (Cx32-WT, 100%; Cx32-R219H, 83.2 ± 2.5%; Cx32-R230C, 61.5 ± 3.3%; Cx32-F235C, 61.3 ± 2.1%). Results are the mean ± S.E. values (n = 3). a and b are significantly (p < 0.05) different as compared with Cx32-WT and each other. D, quantification of the data shown in B. Cx32 co-IP intensity was normalized to CaM IP (Cx32-WT, 100%; Cx32-R219H, 65.3 ± 3.1%; Cx32-R230C, 59.3 ± 3.3%; Cx32-F235C, 53.1 ± 3.6%). Results are the mean ± S.E. values (n = 3). c and d are significantly (p < 0.05) different as compared with Cx32-WT and each other.