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. 2012 Jun 20;287(33):27771–27788. doi: 10.1074/jbc.M112.382572

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Characterization of the Cx32/SAP97 interaction by the Y2H. A, schematic representation of the SAP97 domains and constructs used in the Y2H assay. B, the Saccharomyces cerevisiae yeast strain AH109 was cotransformed with GAL4ad fusion constructs GAL4ad-SH3 (SAP97(569–644)), GAL4ad-HOOK (SAP97(645–700)), GAL4ad-GUK (SAP97(701–764)), GAL4ad-END (SAP97(569–764)), or GAL4ad-SV40 Large T antigen (control) and with GALbd fusion constructs GAL4bd-Cx32CT (Cx32(217–283)) or GAL4bd-pVA3 murine p53 (control). Cotransformants were assayed for growth on nonselective (+His) and selective (−His) media. Abbreviations are as follows; ad, activation domain; bd, DNA-binding domain.