TABLE 1.

Thermodynamic parameters for the interaction of Cx32CT-WT and mutants with calmodulin

Experiments were done in buffer containing 25 mm Tris-HCl, 100 mm KCl, 1 mm β−ME, and 5 mm CaCl₂ at pH 7.0. Experimental errors are as follows: ΔH_b (±5%), ΔK_b (±20%), ΔS_b (±5%), and ΔG_b (±7%).

Protein	ΔHb	n	Kb × 104	ΔGb0	TΔS
	cal/mol		m−1	cal/mol
Cx32CT-WT	−8082	1.01	21.5	−7567	−514.6
Cx32CT-R219H	−7376	1.00	9.6	−7071	−305.0
Cx32CT-R230C	−6825	0.99	6.7	−6851	26.1
Cx32CT-F235C	−4558	1.00	2.6	−6260	1701.9