TABLE 1.

AID-catalyzed deaminations in a lacZa reporter gene on an M13-gapped DNA

WT and mutant AID activity on lacZa gap assay were monitored as an increase in frequency of mutated (white or light blue) plaques over the background mutant frequency without AID (5).

AID mutant	No. of mutant plaques	Total plaques	Mutant frequency
WT	126	6104	206 × 10−4
Class I (catalysis)
H56Y	0	7546	<1.3 × 10−4
E58K	0	9947	<1.0 × 10−4
C87R	3	7177	4.2 × 10−4
C87S	0	5011	<2.0 × 10−4
Class II (substrate interaction)
R24W	0	12,634	<0.8 × 10−4
S83P	0	13,143	<0.8 × 10−4
S85N	0	14,383	<0.7 × 10−4
A111E	6	29,437	2.0 × 10−4
R112C	13	6989	18.6 × 10−4
R112H	1	9488	1.1 × 10−4
L113P	0	9290	<1.1 × 10−4
R174S	46	13,203	34.8 × 10−4
Class III (structural integrity)
M6T	0	5636	<1.8 × 10−4
F11L	1	8415	1.2 × 10−4
F15L	56	13,741	40.8 × 10−4
W80R	2	16,623	1.2 × 10−4
L98R	368	20,851	176.5 × 10−4
L106P	0	4768	<2.1 × 10−4
I136K	0	17,526	<0.6 × 10−4
M139T	0	11,400	<0.9 × 10−4
M139V	0	7935	<1.3 × 10−4
F151S	0	10,511	<1.0 × 10−4
S43P	73	8332	87.6 × 10−4
Class IV (C-terminal deletion)
ΔC10	101	11,840	85.3 × 10−4
ΔC15	226	23,232	97.3 × 10−4
ΔC19	3	11,300	2.7 × 10−4
ΔC20	0	15,304	<0.7 × 10−4
No AID	4	8271	4.8 × 10−4