FIGURE 4.

Calmodulin binding site. Sequence analysis of myosin-XXI to identify potential calmodulin binding sites. A, schematic of the FL-XXI construct. Positions of 16 potential binding sites, determined according to the literature (22, 23, 36), are marked in red. The table shows the sequences and positions of the 16 potential calmodulin binding sites for FL-XXI. Red letters indicate residues consistent with calmodulin-binding motifs according to the literature. The red arrow indicates the most probable site from our calmodulin binding studies. B, table showing a selection of known leucine zipper sequences (37, 38) aligned with the predicted leucine zipper of myosin-XXI. C, table showing the high similarity in sequence of calmodulin found in Xenopus (and used in the experiments in this study) and in L. donovani. Marked in green are the two predicted calcium-binding EF-hand domains.