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. 2012 Jun 20;287(33):27930–27940. doi: 10.1074/jbc.M111.337295

FIGURE 5.

FIGURE 5.

Cross-bridge detachment rates from varying species of myofibrils. Panel A displays the rates of cross-bridge detachment in the absence of ADP at 15 °C from failing human (41 ± 4/s), dog (125 ± 17/s), rabbit (163 ± 8/s), and rat ventricular myofibrils (159 ± 6/s) that were exchanged with TnCIANBDT53C Tn. The TnCIANBDT53C myofibrils in Buffer A + 5 mm EGTA were rapidly mixed with equal volumes of the Buffer A + 5 mm EGTA + 2 mm ATP. Panel B displays the rates of cross-bridge detachment in the presence of 2 mm ADP from failing human (2.3 ± 0.3/s), dog (6.3 ± 0.6/s), rabbit (6.9 ± 0.6/s), and rat ventricular myofibrils (42 ± 3/s) that were exchanged with TnCIANBDT53C Tn at 15 °C. TnCIANBDT53C myofibrils in Buffer A + 5 mm EGTA + 2 mm ADP were rapidly mixed with equal volumes of Buffer A + 5 mm EGTA + 2 mm ATP.