FIGURE 2.
Effect of (−)-EGCG. Synthetic Aβ1–42 (100 μm) was incubated at 25 °C in the absence or presence of different concentrations of EGCG (3, 10, 30, and 100 μm). Samples were taken after 5 h of incubation, diluted 100-fold, and injected over immobilized 4G8 for 2 min (bar), followed by 11 min of dissociation. a, shows the sensorgrams (time course of the SPR signal expressed in resonance units, RU), which could be dissected for the presence of quickly dissociating species (monomers) and slowly dissociating species (oligomers) (see also Fig. 1). b, shows the effects of EGCG on the SPR binding signal caused by each of these components, highlighting a selective inhibitory effect on the oligomeric species.