FIGURE 3.
SPR studies with native Aβ1–42 from C. elegans. a, lysates obtained from CL4176 transgenic C. elegans strain 44 h after the temperature rise (i.e., after induction of oligomeric Aβ1–42 expression) were injected onto immobilized 4G8. Lysates from uninduced CL4176 and CL802 nematodes, which do not express the Aβ1–42 transgene, were used as control. This experiment was carried out twice with similar results. b, lysates from induced CL4176 worms (i.e., 44 h after the temperature rise) were eluted by SEC using a S200 column. Fractions collected at time points corresponding to the elution of monomers and oligomers (void volume) were injected onto immobilized 4G8.