FIGURE 6.

FA formation is regulated by WT (but not S85A) paxillin together with the talin C-terminal tail domain. Cells were cotransfected with HA₃-paxillin (HA₃-Pax; WT or S85A) and GFP-WT talin (A), the N-terminal head domain (B), or the C-terminal tail domain (C and D) for 36 h. Alternatively, cells were transiently transfected with control siRNA (siControl) or siRNA against paxillin (siPaxillin) (E) or cotransfected with paxillin siRNA and HA₃-S85A paxillin (F) for 36 h. The cells were then replated onto collagen I (10 μg/ml)-precoated coverslips for 30 min before staining with anti-HA₃ tag (red, A–C; and green, F), anti-actin (red, E), anti-paxillin (green, E), or anti-vinculin (red, D and F) antibody. Scale bars = 10 μm (A–D and F) and 20 μm (E). The data shown represent three independent experiments.