FIGURE 3.

Quantitative PCR of mRNA in adipose tissues. Total mRNA were isolated from visceral (gonadal) adipose tissues of chow (C) and Western diet-fed (W) APOE3 and APOE4 mice for quantitative PCR analysis of F4/80 (A), NOS2 (B), and TNF-α mRNA (C) levels relative to the expression levels of cyclophilin mRNA. Each point in the display shows average of duplicate measurements in individual animals. The bars in each group represent mean from seven chow-fed APOE3 mice, seven chow-fed APOE4 mice, nine Western diet-fed APOE3 mice, and six Western diet-fed APOE4 mice with significant differences as denoted.