FIGURE 6.

Analysis of cell stress signals in APOE3 and APOE4 macrophages. Peritoneal macrophages isolated from APOE3 (filled bars) and APOE4 (open bars) mice were incubated in the absence or presence of 50 ng/ml LPS or 100 μg/ml oxLDL. Cell proteins were analyzed by Western blot analysis of total and phospho-JNK (A), total and phospho-IRE-1α (B), and total and phospho-PERK (C). Alternative splicing of XBP1 was analyzed based on appearance of alternatively spliced XBP1 mRNA (XBP1s) compared with XBP1 mRNA (D) and alternatively spliced forms of XBP1 protein in cytosol (C) and nucleus (N) (E). The insets in each graph show representative data of n = 3 experiments ± S.E. * denotes significant difference from APOE3 macrophages at p < 0.05.