FIGURE 1.
DMF inhibits dendritic cell maturation. A, BMDCs were stimulated by LPS (100 ng/ml) in the presence or absence of different dosages of DMF (17, 35, and 70 μm). Cytokine secretion was determined by ELISA at 6 and 24 h. Error bars indicate S.E. B, DCs were generated in vivo, purified, and stimulated by LPS (100 ng/ml) in the presence or absence of DMF (70 μm) for 24 h. MHC class II, CD80, and CD86 were then stained for flow cytometric analysis. Numbers indicate the percentages of MHC class IIhigh, CD80-positive, or CD86high. *, p < 0.01. Data are derived from one experiment representative of three independent experiments.