FIGURE 3.

Subcellular localization of GFP-Ac45mutant proteins in transgenic Xenopus melanotrope cells. A, Xenopus melanotrope cells were isolated from the transgenic pituitaries, cultured and examined with live imaging for GFP fluorescence. Note that in the active melanotrope cells the ER is situated near the plasma membrane (53). B, melanotrope cells were fixed and stained with the marker antibodies anti-NaK-ATPase (plasma membrane), anti-calnexin (for the ER) and anti-POMC (for secretory vesicles).