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Excess of cleaved-Ac45 does not affect the inhibition of the spontaneous Ca2+-oscillations in cleaved-Ac45-transgenic Xenopus melanotrope cells. A, Ca2+-oscillations in wild-type (wt) and cleaved-Ac45-transgenic (tg) cells loaded with fura-2 are inhibited in the presence of 0.1 μm apomorphine and reappear after removal of the drug. B, in the presence of 0.1 μm apomorphine low-frequency Ca2+-oscillations occur in ∼25% of the wt and tg cells.
