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. 2012 Jun 13;287(33):27580–27592. doi: 10.1074/jbc.M112.379206

FIGURE 5.

FIGURE 5.

Effect of mutants with single amino acid changes in H. sapiens TAF6 on TAF6-TAF9 and TAF5-TAF6-TAF9 complex formation. A, Coomassie Blue staining of TAF6 and Western blotting of TAF6 and TAF9 from baculovirus-expressed and immunopurified human TAF6-TAF9 human complexes. The different lanes correspond to complexes incorporating full-length human TAF6 either with WT or with single mutations (m9 to m16). Each mutant is described in Table 3. Inputs are displayed on the right side of the panel. B, graph displaying the amount of TAF9 (assessed upon quantification of the TAF9 Western blotting) bound to TAF6 upon complex formation in the presence of the WT or different m9–m16 mutants of TAF6. C, same as in panel A in the case of baculovirus-expressed TAF5-TAF6-TAF9 complexes isolated upon FLAG tag purification of TAF6. Inputs are displayed on the right side of the panel. D, graph displaying different amounts of TAF5 and TAF9 (assessed upon quantification of the TAF5 and TAF9 Western blots) bound to TAF6 upon complex formation in the presence of the WT or different m9–m16 mutants of TAF6. Error bars in panels B and D represent ± S.D.