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. 2012 Jun 20;287(33):27313–27325. doi: 10.1074/jbc.M112.377960

FIGURE 1.

FIGURE 1.

Kinetic and thermodynamic analysis of BMP9 binding to ALK1, ActRIIB, ActRIIA, and BMPRII. A, SPR sensograms of ALK1ECD-Fc, ActRIIBECD-Fc, ActRIIAECD-Fc, and BMPRIIECD-Fc binding to BMP9 at 25 and 37 °C. Raw data (colored lines) were overlaid with a global fit to a 1:1 model with mass transport limitations (black lines). ActRIIAECD-Fc data were analyzed using affinity fit in SigmaPlot. B, kinetic parameters for receptor-ligand interactions. C, comparison of thermodynamic parameters of ALK1ECD-Fc, ActRIIBECD-Fc, and ActRIIAECD-Fc interactions with BMP9 at 25 °C.