Figure 1.
Confocal microscopy of retinal whole mounts. A, Green fluorescent protein (GFP) cells surround the optic nerve head of an eye injected with self-complementary adeno-associated virus type 2 (scAAV2)–chicken β-actin (CBA)–GFP. B, Weak expression of ND4FLAG is seen in a single-stranded (ss) AAV2-CBA-ND4–injected eye. C, Strong ND4FLAG expression is evident in an scAAV2-CBA-ND4–injected eye. D, Retinal cells and nerve fibers show a cytoplasmic and nuclear pattern of GFP expression in scAAV2-CBA-GFP–injected eyes. E, Weak expression of ND4FLAG is evident in eyes injected with ssAAV-CBA-ND4FLAG. F, strong expression of ND4FLAG is seen in eyes injected with scAAV2-CBA-ND4FLAG. Scale bars equal 100 μm.