Figure 2.
(A) Average diffusion coefficient as a function of incubation time for AF488- (black) and AF647-labeled (red) N47A Spc-SH3. (B) Example of lifetime-weighted autocorrelation curve (AF488 channel, 48 h of incubation time) fitted using a single diffusive species (Equation 2). The residuals plot (below) indicates a poor fit.