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. 2012 Jul 26;13(8):9460–9477. doi: 10.3390/ijms13089460

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Conserved regions and phylogenetic analysis of, neutral invertase (NI) protein sequences. (a) Large regions of homology are indicated in boxes I-VIII. Putative catalytic sites (Asp²⁵⁶ and Asp³¹⁰) are highlighted in I and II conserved regions, respectively. The numbers above the alignment represent the neutral invertases sequence of L. temulentum; (b) Phylogenetic dendrogram was generated by the multi-alignment using Molecular Evolutionary Genetics Analysis (MEGA) 4.0 based on identity and rooted with Cyanothece. Sequences are designated by accession numbers and name of organisms from GenBank database.