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. 2012 Jul 26;13(8):9460–9477. doi: 10.3390/ijms13089460

Table 1.

Primer sets used for PCR amplification and expression analysis.

Primer name Forward and reverse primes (5′-3′) Tm (°C)
Ac-sps degenerate primers GGA(G/A)CTTGG(T/A/G/C)CG(T/G)GATTCTGATA
CAGGAAC(A/T/C)TC(A/T)GA(C/T)TG(C/T)TTGTG		 52
Ac-sps specific primers GCTACGGCGAGCCAACCGAGAT
GTGGCCAAGAGCGCCGTCAAC		 60
Ac-susy specific primers 1 GGATGACCGGTCTAAGCCCATCAT
CCATTTCACGCAGTTTGGCGCAT		 60
Ac-ni degenerate primers CAG(T/C)T(T/G/A)CAGAG(C/T)TGGGAGA
GTGTC(A/G)TA(A/C)TA(T/C)TCAGGCCA		 55
Ac-ni specific primers GCTCTGGGGATTTGTCAGTTCAG
CGATCAATCATGCACGAGCCGT		 60
18S rRNA specific primers 2 GCCGGCGACGCATCATTCAA
CGCGCCTGCTGCCTTCCTTG		 60
1

Oligonucleotides designed based on the Ac-susy sequence from pineapple fruit (GenBank ID: DQ438976);

2

Oligonucleotides designed based on the 18S rRNA sequence from pineapple (GenBank ID: D29786).