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. 2012 Jul 26;13(8):9478–9488. doi: 10.3390/ijms13089478

Figure 1.

Figure 1

(A) Dose-dependent effects on Vascular Endothelial Growth Factor (VEGF)-A164 synthesis (Western immunoblotting and its densitometric analysis) elicited by a 24 h of incubation with d-glucose (5.5, 15 and 25 mM) in Vascular Smooth Muscle Cells (VSMC) from lean insulin-sensitive Zucker fa/+ rats (LZR) and obese insulin-resistant Zucker fa/fa rats (OZR); (B) Role on VEGF-A164 synthesis (Western immunoblotting and its densitometric analysis) elicited in VSMC from LZR and OZR by a 24 h of incubation with 19.5 mM l-glucose or mannitol, i.e., with a concentration iso-osmolar to 25 mM d-glucose, taking into account the presence of d-glucose 5.5 mM in the culture medium. Statistical analysis is described in the text.