Figure 4.

Inhibition of Notch-1 signaling pathway by As₂O₃ in breast cancer cells. (A) The Notch-1, Bcl-2 and NF-κB mRNA were detected by RT-PCR in breast cancer cells treated with varied concentrations of As₂O₃ for 72 h; (B) The Notch-1, Bcl-2, and NF-κB proteins were measured by Western blotting analysis in breast cancer cells treated with varied concentrations of As₂O₃ for 72 h; (C) The Notch-1 expression was detected by Western blotting analysis in breast cancer cells treated with 8 μM As₂O₃ for different times.