Figure 3.

Effects of GBR and GABA on H₂O₂-induced reduction of mitochondrial membrane potential. Cells were pre-treated with 10, 50 and 100 μg/mL of GBR and GABA, respectively followed by 250 μM H₂O₂ challenge for 24 h. After incubation, cells were stained with Rhodamine 123 and analyzed by flow cytometer. The reduced fluorescence of Rhodamine 123 was determined as the reduced mitochondrial membrane potential. Results are the mean ± SD in triplicates. * p < 0.01 versus H₂O₂, # p < 0.01 versus control.