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. 2012 Aug 9;13(8):9942–9958. doi: 10.3390/ijms13089942

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Measured values in absorbance units for CA125 standards (0–400 kU/L) (blue), 1:20 diluted P95 serum (green) and 1:20 diluted P95 serum spiked with 80 μg fibronectin (red). The values obtained from P95 serum and spiked P95 serum were used for the Mann-Whitney U test; (B) Standard curve obtained by plotting the detected absorbance units against the kU/L of the CA125 standards. This curve was used to calculate the CA125 concentration of the used samples (C) CA125 concentration measured by a first generation ELISA (M11-like). Green: P95 serum, red: P95 serum spiked with 80 μg fibronectin. The increase in reading is statistically significant (* p < 0.05). Error bars indicate standard deviation of measured values within the respective sample; (D) CA125 concentrations measured by a clinically used second generation ELISA. I: 500 μL P607 serum + 100 μL H₂O; II: 500 μL P607 serum + 100 μL apo-serotransferrin (100 mg/mL); III: 500 μL P607 serum + 100 μL holo-serotransferrin (100 mg/mL). The increase in the reading for CA125 is statistically significant (*) between apo-serotransferrin (II) and unspiked serum (I) and statistically highly significant (** p < 0.01) between holo-serotransferrin (III) and unspiked serum (I). Error bars indicate the standard deviation of the measured values in the respective sample.

(A) Measured values in absorbance units for CA125 standards (0–400 kU/L) (blue), 1:20 diluted P95 serum (green) and 1:20 diluted P95 serum spiked with 80 μg fibronectin (red). The values obtained from P95 serum and spiked P95 serum were used for the Mann-Whitney U test; (B) Standard curve obtained by plotting the detected absorbance units against the kU/L of the CA125 standards. This curve was used to calculate the CA125 concentration of the used samples (C) CA125 concentration measured by a first generation ELISA (M11-like). Green: P95 serum, red: P95 serum spiked with 80 μg fibronectin. The increase in reading is statistically significant (* p < 0.05). Error bars indicate standard deviation of measured values within the respective sample; (D) CA125 concentrations measured by a clinically used second generation ELISA. I: 500 μL P607 serum + 100 μL H₂O; II: 500 μL P607 serum + 100 μL apo-serotransferrin (100 mg/mL); III: 500 μL P607 serum + 100 μL holo-serotransferrin (100 mg/mL). The increase in the reading for CA125 is statistically significant (*) between apo-serotransferrin (II) and unspiked serum (I) and statistically highly significant (** p < 0.01) between holo-serotransferrin (III) and unspiked serum (I). Error bars indicate the standard deviation of the measured values in the respective sample.