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. 2012 Aug 9;13(8):9942–9958. doi: 10.3390/ijms13089942

Table 1.

MASCOT search results for peptides of CA125 from SDS-PAGE gel band IV (Figure 1B) identified by a HCT Ultra 3D Ion Trap mass spectrometer. The score of the peptides written in bold exceed the significance threshold (Expectation ≤ 0.05).

Protein name: Mucin-16
Mass [Da]: 2359682
Score: 577
Matches: 33(5)
Sequences: 21(5)
Sequence Coverage: UniProt Acc.: 1% MUC16_HUMAN
Observed a z b Mr (calc) c Delta d Score e Expect f Peptide
654.42 2 1306.6418 0.1836 84 6.80E-07 R.NSLYVNGFTHR.S
722.45 2 1442.6572 0.2282 68 2.70E-05 K.DGAATGVDAICTHR.L
875.05 2 1747.8628 0.2226 52 0.001 K.LTNDIEELGPYTLDR.N
655.92 2 1309.6085 0.2170 44 0.0069 K.NTSVGPLYSGCR.L
779.93 2 1557.7206 0.1249 41 0.014 K.QEAATGVDTICTHR.V
654.90 2 1307.6259 0.1596 35 0.06 R.DSLYVNGFTHR.S
625.91 2 1249.6204 0.1851 34 0.076 R.GSLYVNGFTHR.T
485.32 2 968.6018 0.0236 32 0.11 R.LTLLRPEK.D
485.32 2 968.6018 0.0236 32 0.12 R.LTLLRPEK.D
485.35 2 968.6018 0.0836 31 0.12 R.LTLLRPEK.D
555.37 2 1108.5877 0.1378 31 0.14 R.LDPLNPGLDR.E
875.00 2 1747.8628 0.1226 29 0.22 K.LTNDIEELGPYTLDR.N
532.38 2 1062.5346 0.2109 28 0.32 K.ELGPYTLDR.N
532.38 2 1062.5346 0.2109 28 0.34 K.ELGPYTLDR.N
642.40 2 1282.5976 0.1879 26 0.46 K.STSVGPLYSGCR.L
598.41 2 1194.5921 0.2134 25 0.49 R.EQLYWELSK.L
596.67 3 1786.8268 0.1614 25 0.51 R.SEKDGAATGVDAICTHR.L
642.40 2 1282.5976 0.1879 25 0.57 K.STSVGPLYSGCR.L
485.39 2 968.6018 0.1636 22 0.7 R.LTLLRPEK.D
399.85 2 797.5123 0.1731 22 0.73 R.VLQGLLR.S
654.90 2 1307.6259 0.1596 24 0.81 R.DSLYVNGFTHR.S
679.95 2 1357.7605 0.1249 23 1 R.GIIELGPYLLDR.G
570.42 2 1138.6022 0.2232 20 1.3 R.VAIYEEFLR.M
662.46 2 1322.7082 0.1973 20 1.3 R.DIQDKVTTLYK.G
532.33 2 1062.5346 0.1109 22 1.4 K.ELGPYTLDR.N
598.41 2 1194.5921 0.2134 21 1.4 R.EQLYWELSK.L
436.95 3 1307.6259 0.2023 20 1.8 R.DSLYVNGFTHR.S
875.00 2 1747.8628 0.1226 20 1.9 K.LTNDIEELGPYTLDR.N
594.27 3 1779.9016 −0.1135 15 5.5 K.QVFHELSQQTHGITR.L
663.40 2 1324.6735 0.1119 11 13 R.LDPTGPGLDRER.L
628.43 2 1254.8064 0.0391 10 18 R.VLQGLLKPLFK.S
a

Observed mass/charge of the peptide in Da;

b

Charge state of the peptide.

c

Theoretical peptide mass in Da;

d

difference between the observed and the theoretical mass in Da;

e

MASCOT score;

f

Expectation of a random match, derived from the MASCOT score;

g

Mass accuracy in parts per million.