Table 2.
Combined MASCOT search results for SEC fractions 15–17 in which CA125 was identified by a LTQ Orbitrap XL mass spectrometer. The score of the peptides written in bold exceed the significance threshold (Expectation ≤ 0.05).
| Observed a | z b | Mr (calc) c | ppm d | Score e | Expect f | Peptide |
|---|---|---|---|---|---|---|
| 748.8278 | 2 | 1495.64 | 0.60 | 52 | 4.60E–05 | K.SYFSDCQVSTFR.S |
| 798.7573 | 3 | 2393.249 | 0.60 | 56 | 5.90E–05 | R.LTLLRPEKDGAATGVDAICTHR.L |
| 570.3081 | 2 | 1138.602 | −0.50 | 44 | 0.0008 | R.VAIYEEFLR.M |
| 399.7638 | 2 | 797.5123 | 1.05 | 37 | 0.00099 | R.VLQGLLR.S |
| 655.812 | 2 | 1309.609 | 0.75 | 42 | 0.0011 | K.NTSVGPLYSGCR.L |
| 721.3632 | 2 | 1440.714 | −1.80 | 39 | 0.003 | K.HGAATGVDAICTLR.L |
| 642.3064 | 2 | 1282.598 | 0.51 | 36 | 0.0039 | K.STSVGPLYSGCR.L |
| 596.814 | 4 | 2383.228 | −0.32 | 38 | 0.0047 | R.LTLLRSEKDGAATGVDAICTHR.L |
| 654.3267 | 2 | 1306.642 | −2.25 | 37 | 0.0058 | R.NSLYVNGFTHR.S |
| 601.8525 | 2 | 1201.689 | 1.02 | 34 | 0.0078 | R.VLQGLLGPMFK.N |
| 523.6422 | 3 | 1567.905 | 0.03 | 30 | 0.011 | R.LTLLRPEKDGVATR.V |
| 607.8801 | 2 | 1213.743 | 1.87 | 27 | 0.014 | R.VLQGLLSPIFK.N |
| 837.1103 | 3 | 2508.312 | −1.12 | 30 | 0.022 | R.LTLLRPEKQEAATGVDTICTHR.V |
| 677.8256 | 2 | 1353.635 | 1.48 | 28 | 0.037 | K.NTSIGPLYSSCR.L |
| 798.1088 | 3 | 2391.306 | −0.49 | 25 | 0.04 | R.LTLLRPEKHGAATGVDAICTLR.L |
| 594.3086 | 3 | 1779.902 | 1.29 | 28 | 0.049 | K.QVFHELSQQTHGITR.L |
| 711.1059 | 4 | 2840.395 | −0.15 | 27 | 0.053 | R.LTSLRPEKDGAATGMDAVCLYHPNPK.R |
| 679.8878 | 2 | 1357.761 | 0.41 | 25 | 0.077 | R.GIIELGPYLLDR.G |
| 628.4118 | 2 | 1254.806 | 2.14 | 12 | 0.086 | R.VLQGLLKPLFK.S |
| 1007.534 | 2 | 2013.053 | 0.59 | 26 | 0.088 | K.LSQLTHGITELGPYTLDR.H |
| 947.8076 | 3 | 2840.395 | 2.15 | 25 | 0.089 | R.LTSLRPEKDGAATGMDAVCLYHPNPK.R |
| 514.2987 | 3 | 1539.873 | 0.68 | 16 | 0.43 | R.LTLLRPEKDGAATR.V |
| 717.8562 | 2 | 1433.697 | 0.39 | 17 | 0.57 | K.VDAICTYRPDPK.S |
| 598.3046 | 2 | 1194.592 | 2.12 | 15 | 0.61 | R.EQLYWELSK.L |
| 607.3171 | 3 | 1818.926 | 1.65 | 17 | 0.69 | K.SPGLNREQLYWELSK.L |
| 555.9742 | 3 | 1664.9 | 0.54 | 13 | 1.2 | R.RVDRVAIYEEFLR.M |
| 514.2987 | 3 | 1539.873 | 0.68 | 10 | 1.8 | R.LTLLRPEKDGAATR.V |
| 477.5088 | 4 | 1906.01 | −1.85 | 6 | 6.3 | K.NTSVGPLYSGCRLTLLR.S |
| 862.958 | 2 | 1723.903 | −1.02 | 5 | 7.8 | K.DPEILSWTIPPSIEK.T |
| 647.5736 | 4 | 2586.261 | 1.71 | 5 | 8.8 | K.HEAATGVDTICTHRVDPIGPGLDR.E |
| 702.6927 | 3 | 2105.058 | −0.61 | 4 | 11 | K.KDGAATKVDAICTYRPDPK.S |
| 848.0892 | 3 | 2541.237 | 3.53 | 1 | 19 | K.SKLSLTPGLMETSISEETSSATEK.S |
a
Observed mass/charge of the peptide in Da;
b
Charge state of the peptide;
c
Theoretical peptide mass in Da;
d
Mass accuracy in parts per million;
e
MASCOT score;
f
Expectation of a random match, derived from the MASCOT score.