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. 2012 Aug 23;13(8):10594–10607. doi: 10.3390/ijms130810594

Figure 2.

Figure 2

Effect of sCLU knockdown on the invasive behavior of HCCLM3 cells. (A) HCCLM3 cells were treated with 5.25, and 50 μg/mL antisense oligonucleotide (ASO) against sCLU (OGX-011) or MM (mock control) for 48 h. sCLU expression was detected by Western blotting; (B) Histogram represents the density of bands in A normalized with β-actin; (C) Effect of sCLU knockdown on cells subjected to invasion assays using a two-chambered invasion apparatus. The histogram shows percent inhibition of HCCLM3 cell invasion. The experiment was done in triplicate and the value obtained from MM-treated cells was set at 100%. Each bar represents mean ± SE (n = 3); * p < 0.05, ** p < 0.01.