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. 2012 Aug 23;13(8):10594–10607. doi: 10.3390/ijms130810594

Figure 4.

Figure 4

Effect of sCLU knockdown in HCCLM3 cells on MMP-2 gene expression. (A) Representative images showing the expression of MMP-2 mRNA, as determined by RT-PCR; (B) Western blot analysis to evaluate pro-MMP-2 protein expression in sCLU-knockdown HCCLM3 cells. Blot was reprobed with β-actin antibody to verify the equal loading of proteins; (C) Gelatin zymogram showing activity of pro-MMP-2 and active MMP-2 in OGX-011 and MM treated HCCLM3 cells. Columns, mean of quadruple experiments; bars, SD. * p < 0.05.