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. 2012 Aug 23;13(8):10594–10607. doi: 10.3390/ijms130810594

Figure 5.

Figure 5

Effect of sCLU overexpression on MMP-2 expression and activity. (A) HepG2 cells were transfected with pc.DNA3.1-sCLU or pcDNA3.1 for 36 h. Representative images showing expression of MMP-2 mRNA in transfected cells, as determined by RT-PCR; (B) Western blot analysis to evaluate pro-MMP-2 protein expression in pc.DNA3.1-sCLU or pc.DNA3.1-transfected HepG2 cells. Blot was reprobed with β-actin antibody to verify the equal loading of proteins; (C) Gelatin zymogram showing activity of pro-MMP-2 and active MMP-2 in pc.DNA3.1-sCLU or pc.DNA3.1-transfected HepG2 cells. Columns, mean of quadruple experiments; bars, SD. * p < 0.05.