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. Author manuscript; available in PMC: 2012 Aug 31.
Published in final edited form as: J Am Chem Soc. 2005 Jun 22;127(24):8600–8601. doi: 10.1021/ja051462l

Table 1.

Oligonucleotide Coverages for Various Thiol SAM Deposition and Oligonucleotide Coupling Conditions

thiol solution coupling solution coverage (oligos/cm2) hybridization efficiency
5 mM N3(CH2)11SH standard 1.1 ± 0.1 × 1013 24 ± 11%
5 mM N3(CH2)11SH no acetylene on oligo 2.3 ± 0.8 × 1010 N/A
5 mM N3(CH2)11SH standard + 13 × 2-methoxy propylamine 1.1 ± 0.2 × 1013 N/A
5 mM N3(CH2)11SH standard + 13 × acrylonitrile 1.1 ± 0.1 × 1013 N/A
<0.05 mM N3(CH2)11SHa
4.95 mM CH3(CH2)7SH		 standard 3.6 ± 0.2 × 1012 97 ± 7%
0.05 mM N3(CH2)11SH
4.95 mM HO(CH2)6SH		 standard 1.1 ± 0.1 × 1012 96 ± 13%
a

Azide thiol had partially oxidized to the azide disulfide, which is known to adsorb less avidly than the azide thiol.