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. 2012 Sep 1;23(17):3420–3428. doi: 10.1091/mbc.E12-02-0107

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© 2012 Pusnik et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — pATOM36 is essential for normal growth of procyclic and bloodstream forms of T. brucei. (A) Growth curve of an uninduced and tet-induced procyclic pATOM36 RNAi cell line. Right, Northern blot of total RNA isolated from uninduced and induced (2 d) RNAi cell lines probed for pATOM36 mRNA. The rRNA region of the ethidium bromide–stained gel (EtBr) was used as a loading control. (B) As in A, but results are for an uninduced and tet-induced pATOM36 RNAi cell line of the bloodstream form.