FIGURE 2:

ADAM12L interacts with ILK. Cell extracts from (A) Cos7 cells transfected with the long form of ADAM12, ADAM12L, or (B) HSCs were immunoprecipitated with anti-ADAM12 or anti-ILK (IP) and immunoblotted with anti-ILK or anti-ADAM12 antibodies (IB). (C) Cos7 cells were transfected either with ADAM12L or the ADAM12-Δcyto cytoplasmic tail deletion or the short ADAM12S secreted form. Cell extracts were immunoprecipitated with anti-ILK and immunoblotted with anti-ADAM12 (right). Left, immunoblots performed on crude extracts as controls. (D) Cos7 cells transfected with ADAM12L were immunoprecipitated with anti-ILK and immunoblotted (IB) with anti-PINCH, anti-parvin, or anti-ADAM12 antibodies. (E) ADAM12L, ADAM12S, and ADAM12-Δcyto were expressed as His-tagged fusion proteins, and binding assays were performed by incubating His-ADAM12 bound to nickel-agarose with 1 μg of purified ILK. Interacting proteins were immunoblotted with anti-ILK and anti-ADAM12 antibodies.