Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Sep 1;23(17):3461–3472. doi: 10.1091/mbc.E11-11-0918

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Leyme et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — ADAM12L and ILK are recruited to focal adhesion structures upon β1 integrin stimulation. HSCs were cultured on plastic dishes (–) or on dishes coated with type I collagen (+) overnight in medium containing 2% FBS. Cell were immunostained with antibodies against anti-ADAM12, anti-ILK, anti-vinculin, and anti-ITGB1 as indicated, followed by tetramethylrhodamine isothiocyanate– or fluorescein isothiocyanate–labeled secondary IgG. Representative fields are shown. Colocalization results in yellow cellular staining. (A) Localization of ADAM12 and ILK. (B) Localization of β1 integrin (ITGB1) and ADAM12. (C) Localization of vinculin and ADAM12. (D) Localization of ILK and β1 integrin. (E) HSCs were preincubated with anti–β1 integrin blocking antibodies or control mouse IgG1 before seeding on type I collagen. Scale bars, 10 μm.