TABLE 3:
Ashbya strains used in this study.
Strain | Relevant genotypea | Source |
---|---|---|
wild-type | Δleu2Δthr4 | Altmann-Johl and Philippsen, 1996 |
AG120 | Agelm1Δ::GEN3/AgSHS1-GFP-NAT1 | DeMay et al., 2009 |
AG124 | AgSHS1-GFP-GEN3 | Helfer and Gladfelter, 2006 |
AG208 | pAGB141 [pAg CDC11a-mCHERRY-NAT1]/AgSEP7-GFP-GEN3 | This study |
AG442.1 | pAGB088 [pAgSHS1-GFP-Gen] | This study |
AG495.1 | AgCDC11a-TAP-GEN3 | This study |
AG539.2 | AgSHS1ΔC-GFP-GEN3 | This study |
AG623 | AgSHS1ΔCC-GFP-GEN3 | This study |
AG632.1 | Agelm1Δnat/AgSHS1ΔC-GFP-GEN3 | This study |
AG643.1 | AgSHS1-GFP-GEN3 9A | This study |
AG647.1 | pAGB412.1 [pAgSHS1ΔC-GFP-GEN3] | This study |
AG664.1 | pAGB141 [pAg CDC11a-mCHERRY-NAT1]/AgSHS1ΔCC-GFP-GEN3 | This study |
aWith the exception of plasmidic strains (in brackets), all analyzed mycelia were homokaryotic (all nuclei have the same genotype).