FIGURE 6:

The deafness mutant, Cx26V37I, but not Cx26A40G, forms functional gap junction channels. (A) Representative family of gap junction current traces from pairs of HeLa cells transfected with Cx26-GFP, Cx26V37I-GFP, or Cx26A40G-GFP recorded by double whole-cell patch-clamp. Voltage-clamp steps were applied to voltages between −100 and +10 mV in 10-mV increments from a holding potential of −60 mV (top panel). (B) The bars in the graph depict the average ± SEM of gap junction conductance in pairs of untransfected HeLa cells (Un) or HeLa cells transfected with Cx26-GFP (in the absence or in the presence of 18-βGA), Cx26V37I-GFP, or Cx26A40G-GFP.