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. 2012 Sep 1;23(17):3299–3311. doi: 10.1091/mbc.E11-12-1058

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© 2012 Jara et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Hemichannels formed by the Cx26 deafness mutants, V37I and A40G, are nonfunctional. (A–C) Graphs show the time course of uptake of YO-PRO-1 (A), ethidium (B), and Neurobiotin (C) in HeLa cells expressing Cx26-GFP (in the absence or in the presence of 18-βGA or La⁺³), Cx26V37I-GFP, or Cx26A40G-GFP under conditions of free extracellular calcium (to induce the opening of hemichannels).