TABLE 1:
Homodimerization of expressed connexin TM domains in the bacterial plasma membrane as determined by TOXCAT analysis.
| Growth in | |||
|---|---|---|---|
| Domain | Sequence | Ampicillin | Chloramphenicol |
| Cx26 TM1 | GKIWLTVLFIFRIMILVVAA | + | 120 μg/ml |
| Cx26 TM2 | LWALQLIMVSTPALLVAMHV | + | − |
| Cx26 TM3 | IFFRVIFEAVFMYVFYIMYNGFF | − | |
| Cx26 TM3A | LWWTYTTSIFFRVIFEAVF | − | |
| Cx26 TM4 | VFTVFMISVSGICILLNITEL | − | |
| Cx43 TM1 | TAGGKVWLSVLFIFRILLLGTAV | + | − |
| Cx26 TM1 DelVVAA | GKIWLTVLFIFRIMIL | + | − |
| Cx26 TM1 GTAV | GKIWLTVLFIFRIMILGTAV | + | − |
| Cx26 TM1 V37I | GKIWLTVLFIFRIMILIVAA | + | 80 μg/ml |
| Cx26 TM1 A40G | GKIWLTVLFIFRIMILVVAG | + | − |
Bacteria were transformed with TOXCAT constructs containing the indicated TM domains and tested for growth in 100 μg/ml ampicillin and increasing concentrations of chloramphenicol (20–120 μg/ml). Bacterial growth is indicated as positive (+); lack of bacterial growth is indicated as negative (−). The maximal concentration of chloramphenicol at which there was bacterial growth is indicated.