FIGURE 3:

Collagen but not fibronectin also reduces intracellular doxorubicin content as measured by fluorescence microscopy. Jurkat (A) and HSB2 (B) cells were preactivated with collagen (Col), fibronectin (Fn), or left unstimulated (NT) for 4 h and then were treated or not with doxorubicin (Dox) for 2 h. The cells were washed, fixed, and mounted between slides and cover slips and were observed by phase contrast and by using a red excitation fluorescence filter. The results are representative of three independent experiments.