Table 1.
HLA-DQB1 alleles inferred from the presence or absence of 9 possible probe-pairing ligation products.
| Allele Group | Probes amplified | Alleles Amplifieda | ||||
|---|---|---|---|---|---|---|
| HLA-DQB1*02 | 1, 2 | DQB1*02:01 | DQB1*02:04 | |||
| DQB1*02:02 | DQB1*02:05 | |||||
| DQB1*02:03 | DQB1*02:06 | |||||
|
| ||||||
| HLA-DQB1*03 | 1, 5 | DQB1*03:01 | DQB1*03:09 | DQB1*03:16 | DQB1*03:23 | DQB1*03:31 |
| DQB1*03:02 | DQB1*03:10 | DQB1*03:17 | DQB1*03:24 | DQB1*03:32 | ||
| DQB1*03:03 | DQB1*03:11 | DQB1*03:18 | DQB1*03:26 | DQB1*03:33 | ||
| DQB1*03:04 | DQB1*03:12 | DQB1*03:19 | DQB1*03:27 | DQB1*03:34 | ||
| DQB1*03:05 | DQB1*03:13 | DQB1*03:20 | DQB1*03:28 | DQB1*03:35 | ||
| DQB1*03:07 | DQB1*03:14 | DQB1*03:21 | DQB1*03:29 | DQB1*03:36 | ||
| DQB1*03:08 | DQB1*03:15 | DQB1*03:22 | DQB1*03:30 | |||
|
| ||||||
| HLA-DQB1*04 | 1, 3 | DQB1*04:01 | DQB1*04:04 | DQB1*04:07 | ||
| DQB1*04:02 | DQB1*04:05 | DQB1*04:08 | ||||
| DQB1*04:03 | DQB1*04:06 | |||||
|
| ||||||
| HLA-DQB1*05 | 4 | DQB1*05:01 | DQB1*05:04 | DQB1*05:07 | DQB1*05:10 | |
| DQB1*05:02 | DQB1*05:05 | DQB1*05:08 | DQB1*05:11 | |||
| DQB1*05:03 | DQB1*05:06 | DQB1*05:09 | ||||
|
| ||||||
| HLA-DQB1*06A | 5b, 6, 7 | DQB1*06:01 | ||||
| DQB1*06:35 | ||||||
|
| ||||||
| HLA-DQB1*06B | 5b, 7 | DQB1*06:02 | DQB1*06:16 | DQB1*06:23 | DQB1*06:29 | DQB1*06:40 |
| DQB1*06:10 | DQB1*06:18 | DQB1*06:24 | DQB1*06:33 | DQB1*06:43 | ||
| DQB1*06:11 | DQB1*06:19 | DQB1*06:26N | DQB1*06:37 | |||
|
| ||||||
| HLA-DQB1*06C | 5b,7,9 | DQB1*06:03 | DQB1*06:27 | DQB1*06:31 | ||
| DQB1*06:08c | DQB1*06:28 | DQB1*06:32 | ||||
| DQB1*06:14d | DQB1*06:30 | DQB1*06:41 | ||||
|
| ||||||
| HLA-DQB1*06D | 5b,8,9 | DQB1*06:04 | DQB1*06:21 | DQB1*06:36 | ||
| DQB1*06:07 | DQB1*06:25 | DQB1*06:38 | ||||
| DQB1*06:17 | DQB1*06:34 | DQB1*06:39 | ||||
|
| ||||||
| HLA-DQB1*06E | 5b,8 | DQB1*06:05 | DQB1*06:15 | |||
| DQB1*06:09 | DQB1*06:22 | |||||
| DQB1*06:12 | DQB1*06:42 | |||||
a
Probe amplifications for each allele were determined theoretically based on known sequences. However, alleles in boldface have been experimentally verified to behave as predicted in this assay.
b
Probe pair 5 amplified significantly less in the presence of *06 alleles compared to *03 alleles, likely due to a polymorphism away from the ligation site on the 5R probe.
c
The complete exon 2 sequence of DQB1*06:08:01 is unknown, and unknown variation in this allele may disrupt the binding of probe 7.
d
Allele DQB1*06:14:02 may not amplify probe 9 due to a single nucleotide mismatch in the left hybridizing sequence.