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. Author manuscript; available in PMC: 2012 Aug 31.
Published in final edited form as: Tissue Antigens. 2011 Jul 18;78(4):275–280. doi: 10.1111/j.1399-0039.2011.01737.x

Table 2.

Sequence of oligonucleotides probes and primers used.

Probe Sequence (5′-3′)a,b Size (nt)e
4L gtggcagggcgctacgaacaatACGGAGCGCGTGCGG
4R P-GGTGTGACCAGACACATCTATAACCgcgccagcaagatccaatctaga 85
3L gtggcagggcgctacgaacaatCTCCAGGATGTCCTTCTGGCTA
3R P-TTCCAGTACTCGGCGTCAAGCTgcgccagcaagatccaatctaga 89
6L gtggcagggcgctacgaacaatACATCCTGGAGAGGACCCGA
6R P-GCGGAGTTGGACACGGTGCCTATAGCGAgcgccagcaagatccaatctaga 93
5L gtggcagggcgctacgaacaatGTCTGCACACCGTGTCCAACT
5R P-CCGCCCGGGTCCTCTCCCCTATAGCGACTTAgcgccagcaagatccaatctaga 97
8L gtggcagggcgctacgaacaatCCTATCAGGATCCCGCGGTACC
8R P-CCACCTCGTAGTTGTGTCTGCACCTATAGCGACTgcgccagcaagatccaatctaga 101
7L gtggcagggcgctacgaacaatAGACACAACTACGAGGTGGCGTT
7R P-CCGCGGGATCTTGCAGACCTATAGCGACTTACGGACGgcgccagcaagatccaatctaga 105
1L gtggcagggcgctacgaacaatCCTATCAGACACAACTACCAGTTGGAGCT
1R P-CCGCACGACCTTGCAGCCCTATAGCGACTTACGGAgcgccagcaagatccaatctaga 109
cC1L gtggcagggcgctacgaacaatCCTATAGCGACAGCAGCCACCTATGGGAATGATG
C1R P-GGTCAAGTTAACCAAGGCAATCATATGATGactagcgccagcaagatccaatctaga 113
cC2L gtggcagggcgctacgaacaatCTCCAGAGAACTTTACCAGCTGTGGGTTCATGCA
C2R P-GCAGATCCAGAAGGGTAGTTATCCTGATGCGATTTTgcgccagcaagatccaatctaga 115
2L gtggcagggcgctacgaacaatCCTATAGCGACTTACGGCGTGCGTCTTGTGAGCAGAA
2R P-GCATCTATAACCGAGAAGAGATCGTGCCTATAGCGACgcgccagcaagatccaatctaga 119
9L gtggcagggcgctacgaacaatCTATAGCGACTTAGAGCGCGTGCGTCTTGTAACCAGAC
9R P-ACATCTATAACCGAGAGGAGTACGCGCCCTATAGCGACTAgcgccagcaagatccaatctaga 123
PCR-L d6FAM-GTGGCAGGGCGCTACGAACAAT
PCR-R TCTAGATTGGATCTTGCTGGCGC
a

For the probes (labeled 4L to 9R) the lower-case letters indicate PCR primer sequences, bolded capital letters indicate spacer sequences, and non-bold capital letters indicate DNA hybridization sequences.

b

The 5′ end of the right probe is phosphorylated (P) to allow ligation to the 3′ end of the left probe.

c

C1 and C2 are positive control probes that hybridize to genomic regions without known variants.

d

The left PCR primer (PCR-L) was labeled at the 5′ end with 6-carboxyfluorescein (6FAM).

e

The combined, ligated size in nucleotides (nt) of each probe pair is given in the rightmost column.