Table 4.
Cost analysis of this methoda
| Step | Reagent | Cost/Sample |
|---|---|---|
| Hybridization | KCl | <$0.01 |
| Tris-HCl | <$0.01 | |
| EDTA | <$0.01 | |
| Plate | $0.06 | |
| Plate Seal | <$0.01 | |
|
| ||
| Ligation | Taq DNA Ligase & buffer | $0.45 |
|
| ||
| PCR | dNTPs | $0.12 |
| Phire Polymerase | $3.52 | |
| Plate | $0.06 | |
| Plate Seal | <$0.01 | |
|
| ||
| Fragment Analysis | Formamide | $0.02 |
| ROX DNA ladder | <$0.01 | |
| Foil Seal | $0.04 | |
| Fragment Plate | $0.12 | |
| Analysis Charge | $1.20 | |
|
| ||
| 1 time costs | Probes | ~$360 |
| PCR Primers (FAM labeled) | ~$160 | |
|
| ||
| Summary | Cost per sample | $5.59 |
| -PCR costs/sample | $3.70 | |
| -Other costs/sample | $1.89 | |
| One time costs | $520 | |
a
Costs omitted from this table include: pipette tips, labor, basic laboratory equipment (thermocycler, pipettes, computer).