Table 1.
Outcome of different subculturing steps
| |
|
Number of strains |
|
|
|---|---|---|---|---|
| Subculturing steps | Days of incubation | API 20E identified Ye | BT1A | |
| 1. PSB (i) |
0 (3h) |
3 |
0 |
|
| 2. TSBY (ii) |
1 |
7 |
4 |
|
| 3. TSBY+KOH-treatment (ii) |
1 |
17 |
8 |
|
| 4. TSBY+KOH-treatment (ii) |
2 |
13 |
9 |
|
| 5. PSB+MRB (i) |
8+4 |
18 |
8 |
|
| 6. PSB (i) |
21 |
22 |
5 |
|
| Total | 80 | 34 | ||
The number of isolates identified as Yersinia enterocolitica (Ye) by API 20E and biotyped as 1A (BT1A) found at different subculturing (CIN) steps during the culture procedures applied.
(i), (ii) refers to the different pathways, shown in Figure 1. PSB, Phosphate-buffered saline containing 2% sorbitol and 0.15% bile salts; TSBY, Tryptone-soya broth supplemented with yeast; KOH, Potassium hydroxide solution; MRB, Modified-Rappaport-broth.